Tested applications
IHC-P, IHC-Fr, WB, ICC/IF
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Overview
Product name
Anti-CTHRC1 antibody [16D04]
See all CTHRC1 products （5） ...
Description
Mouse monoclonal [16D04] to CTHRC1
Tested applications
IHC-P, IHC-Fr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Immunogen
Bacterial expressed full-length rat CTHRC1, reduced and non-reduced
Epitope
ab72527 recognises an epitope located at N Terminal region of CTHRC1 between Serine 32 and Valine 49
General notes
P.I. 7.5-7.8
Properties
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 150mM PBS
Purification notes
Purified from ascites.
Research areas
Cardiovascular >> Vasculature >> Vasculature Markers >> Arterial
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Western blot - CTHRC1 antibody [16D04] （ab72527）![Western blot - CTHRC1 antibody [16D04] （ab72527） image](https://img73.foodjx.com/bb33f2c9b6dc3b85eb515dd667773c4aec020bb7d69a45b163b3034bf3de10d4c9bd6a90bff3b691.jpg)
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Immunocytochemistry/ Immunofluorescence - CTHRC1 antibody [16D04] （ab72527）![Immunocytochemistry/ Immunofluorescence - CTHRC1 antibody [16D04] （ab72527） image](https://img73.foodjx.com/bb33f2c9b6dc3b85eb515dd667773c4a90161c86e3f4be0daa948ad2555abcc5c6ad09f128a0a0d1.jpg)
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Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-CTHRC1 antibody [16D04] （ab72527）![Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - [16D04] （ab72527） image](https://img72.foodjx.com/bb33f2c9b6dc3b85eb515dd667773c4a25bbf9d21b27a63a3df04bd7cee74fb4fe96fedbc6a7404a.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab72527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Target
Function
May act as a negative regulator of collagen matrix deposition.
Tissue specificity
Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells.
Sequence similarities
Contains 1 collagen-like domain.
Post-translational
modifications
N-glycosylated.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ96CG8The UniProt Consortium
The Universal Protein Resource （UniProt） in 2010
Nucleic Acids Res. 38:D142-D148 （2010）.
Information by UniProt
Alternative names
- Collagen triple helix repeat containing 1 antibody
- Collagen triple helix repeat containing protein 1 antibody
- Collagen triple helix repeat-containing protein 1 antibody
- CTHR1_HUMAN antibody
- Cthrc1 antibody
- NMTC1 protein antibody
- Protein NMTC1 antibody
see all
Database links
- Entrez Gene: 115908 Human
- Entrez Gene: 68588 Mouse
- Entrez Gene: 282836 Rat
- Omim: 610635 Human
- SwissProt: Q96CG8 Human
- SwissProt: Q9D1D6 Mouse
- SwissProt: Q8CG08 Rat
- Unigene: 405614 Human
- Unigene: 41556 Mouse
- Unigene: 9468 Rat
see all
[16D04] images:
Western blot - CTHRC1 antibody [16D04] （ab72527）
Lane 1 : [16D04] （ab72527） at 1/10000 dilution
Lane 2 : [16D04] （ab72527） at 1/20000 dilution
Lane 3 : [16D04] （ab72527） at 1/40000 dilution
Lane 1 : non reduced CHo full lenght CTHRC1 conditioned media
Lane 2 : non reduced CHo full lenght CTHRC1 conditioned media
Lane 3 : non reduced CHo full lenght CTHRC1 conditioned media
Predicted band size : 27 kDa
Observed band size : 55,70 kDa （why is the actual band size different from the predicted?）
Immunocytochemistry/ Immunofluorescence - CTHRC1 antibody [16D04] （ab72527）
CHO cells transduced with a full-length CTHRC1 virus. CTHRC1 is shown in red and nuclei in Blue. ab72527 dilution is 1:1000
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - [16D04] （ab72527）
IHC image of ab72527 staining in human melanoma formalinfixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA （pH9, epitope retrieval solution 2） for 20 mins. The section was then incubated with ab72527, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems （automated and non-automated） customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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