Dynabeads® Human T-Activator CD3/CD28 is for the activation and expansion of human T cells for research purposes.

With Dynabeads® Human T-Activator CD3/CD28 you get:

• Activation of T cells without the need of feeder cells
• Activated cells that retain in vivo-like function
• Maximal reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1000-fold T cell expansion

How Dynabeads® Human T-Activator CD3/CD28 works:
Dynabeads® Human T-Activator CD3/CD28 offers a simple method for activation and expansion of T cells that does not require feeder cells （antigen-presenting cells） or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T-cell activation and expansion. Expansion of the T-cell population can be stimulated using View Morerecombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

For expansion of antigen specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.

Starting samples
Start with mononuclear cells （MNCs）/peripheral blood mononuclear cells （PBMCs） from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. For an overview over the different Dynabeads® available for cell isolation, look here.

Downstream applications
The activated T cells can be analyzed shortly after activation （for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.）. T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

For research use only. Not for use in diagnostic procedures. View Less